16S rRNA gene sequencing of colon content samples from mouse treated with or without κ-carrageenan
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https://figshare.com/articles/dataset/16S_rRNA_gene_sequencing_of_colon_content_samples_from_mouse_treated_with_or_without_-carrageenan/17148965
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资源简介：
The fecal
samples collected from the colon lumen of mice at necropsy were used to extract microbial genomic DNA
using CTAB method. Gel electrophoresis was used to monitor the concentration
and evaluate the purity of DNA. The hypervariable V3-V4 regions of the 16S rRNA
gene were sequenced with specific primers (forward primer, 341F:
CCTAYGGGRBGCASCAG; reverse primer, 806R:
GGACTACNNGGGTATCTAAT) tagged with barcodes. The PCR reactions were
carried out with Phusion® High-Fidelity PCR Master Mix (New England Biolabs).
The sequencing library was generated using TruSeq® DNA PCR-Free Sample
Preparation Kit (Illumina, USA) and was sequenced on an Illumina NovaSeq
platform.
创建时间：
2021-12-10
