Three-dimensional brain-like microenvironments facilitate the direct reprogramming of fibroblasts into therapeutic neurons. Three-dimensional brain-like microenvironments facilitate the direct reprogramming of fibroblasts into therapeutic neurons

We report differential mRNA expression levels in primary mouse embryonic fibroblasts, primary neurons, and induced neuronal cells generated via direct reprogramming method. The induced neuronal cells were cultured on laminin or decellularized brain ECM. Overall design: Examination of mRNA expression levels in 4 different groups