Cell segmentation software key feature comparison.

Considered are only tools with a graphical user interface since end users should not need programming expertise. Non deep learning segmentation methods may require expert knowledge for parametrization and are not state-of-the-art anymore. Data format support does not necessarily mean that each image can be processed: if no data management system (DMS) with metadata support is used, e.g., the channel dimension can be the first or the last dimension for.tif files, and the method may have requirements on the channel dimension position. ━: feature not fulfilled/supported, : feature only fulfilled/supported with restrictions, ✔: feature fulfilled/supported.