RNA pull-down analysis of hsa_circ_0001400 in 293T cells. RNA pull-down analysis of hsa_circ_0001400 in 293T cells

293T cells are transfected with hsa_circ_0001400 expression plasmid vectors for 24 hours. Cells are then incuvated with Psoralen, exposed to long-wave UV for 10 minutes, and total RNA was extracted. Biotynilated DNA oligos sense (control) or antisense of circ_0001400 are added and circRNA-RNA complexes are enriched using streptavidin beads. RNA was cleaned and exposed to short-wave UV and subjected for RNA-Seq. Overall design: Triplicates of 293T cells are pulled-down for circ_0001400 using antisense DNA probes or control.