Ethanol abolishes vigilance-dependent astroglia network activation in mice by inhibiting norepinephrine release

This collection of data sets was obtained during a study of the effect of acute ethanol intoxication on vigilance-dependent astroglia network activation in mice. The main findings have been that ethanol inhibits astroglia activation by inhibiting vigilance-dependent norepinephrine release. This leads to a failure of activation of alpha1A-adrenergic receptors on astroglia. Further, this study has revealed that ethanol inhibition of cerebellar Bergmann glia Ca2+ activation does not account for ataxic motor behavior, but may rather contribute to cognitive deficits.

Methods
The majority of data were acquired using two-photon Ca2+ imaging in awake behaving mice on a motorized linear treadmill, or in acute cerebellar slices. The data have been processed using MATLAB scripts. The study also includes quantification of motor coordination using the Catwalk XT system.