Saliva cell type DNA methylation reference panel for epidemiology studies in children

We profiled the DNA methylation of saliva cell types, to develop a tool for epidemiologic studies. Saliva was collected from 22 children, 21 participants with samples usable for DNA methylation, and sorted into immune and epithelial cells, using size exclusion filtration and magnetic bead sorting. DNA methylation was measured using the Illumina MethylationEPIC BeadChip. Saliva immune and epithelial cells have distinct DNA methylation profiles, which can influence whole saliva epidemiologic measures. 60 samples from 21 individuals: 18 large cell fractions, 20 CD45+ cell fractions, 18 whole saliva, 4 Oragene kit