Human immunodeficiency virus Variation. Human immunodeficiency virus

The goal of the study is the development of a high-throughput sequencing-based mismatch detection assay (Diff-seq). Diff-seq relies on the activity of Surveyor endonuclease on mismatched molecules, generated after cross-annealing of a complex pool of DNA fragments. Sequencing libraries prepared after enrichment for Surveyor-cleaved molecules result in increased coverage at the variant sites.