Systematic evaluation of conditional gene essentiality changes across 4 yeast strains

The extent by which different genetic backgrounds affect conditional gene essentiality is understudied. Here, 4 whole-genome knockout libraries were screened to systematically record changes in conditional gene essentiality. Gene expression changes between the reference strain and the other three were measured to gain more knowledge on the molecular determinants of these changes. A total number of 8 samples were analysed, each one in triplicate. The 8 total samples included the four strains grown in two different conditions; regular media and in exposed to 20mM of Caffeine for 4 hours. All samples were harvested when cultures reached O.D. of 0.4.