Results of RNA-seq of ribosome fractions from control and TSC2 KO Day37 organoids

mTOR pathway is a major regulator of translation in eukaryotic cells. Here we investigate the impact of mTOR overactivation through TSC2 KO on the translatome of brain organoid tissue. Day37 organoids were dissociated and the lysate was used for polysome profiling. Monosome, low-polysome and high-polysome fractions were separately collected and the RNA associated with these fractions was isolated and used for RNA-seq