Carbenoxolone inhibition of the light-evoked Ca2+ response in solitary ipRGCs in vitro is difficult to reverse.

Complete Recovery: ipRGCs demonstrating light-evoked Ca2+ responses during carbenoxolone (CBX) washout (45 min) that were ≥100% of the light-evoked Ca2+ response immediately prior to drug treatment were classified as demonstrating complete recovery (n = 5). (time elapsed when complete recovery was noted)Partial Recovery: ipRGCs demonstrating light-evoked Ca2+ responses during CBX washout (45 min) that were greater than the light-evoked Ca2+ response during CBX application and between 50–90% of the light-evoked Ca2+ response immediately prior to drug treatment were classified as demonstrating partial recovery (n = 7).No Recovery: ipRGCs demonstrating light-evoked Ca2+ responses during CBX washout (45 min) that remained 2+ response immediately prior to drug treatment were classified as demonstrating no recovery (n = 8).No Inhibition: ipRGCs demonstrating light-evoked Ca2+ responses in the presence of CBX that were between 95–120% of the preceding light-evoked Ca2+ response in the absence of CBX were classified as demonstrating no inhibition (n = 5).