AspC-mediated metabolism specially affects cell cycle parameters.

Exponentially growing cells in ABTG medium (AB minimal salts supplemented with thiamin and glucose) were treated with rifampicin and cephalexin, fixed in 70% ethanol and then analyzed by flow cytometry as described in Materials and Methods. Average number of origins per cell (A. O.) was calculated using a software provided by BD and cell size of exponentially growing cells in the medium above was determined by fluorescent microscopy. Each calculation includes more than 100 cells. The values are average of three individual experiments and standard errors are given.