Confirmation of protein phosphorylation during 12 hour SDL.

12 hour SDL samples were fractionated by 2D PAGE and then hyper-phosphorylated spots previously identified by mass spectrometry as HSP90 alpha, Hsp90 beta and nascent polypeptide associated complex alpha subunit were subjected to Nano LC mass spectrometry to detect phosphopeptides as outlined in the materials and methods. It should be noted that for some peptides there may be more than one possible phosphorylation site, so we have included all possibilities and known phosphorylation sites within the peptide has been indicated with an *. For some peptides there are more than one possible phosphorylation site and so all matched options are presented. *Indicates a known reported phosphorylation site [43], [61].