Kinetic constants of wild-type enzymes and selected variants toward galactomannan, mannohexaose (M6) and mannopentaose (M5).
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The kinetic parameters were determined at 40°C in sodium acetate buffer 50 mM, pH 5.2 as described in the Methods section. Paired t test was used to compare the kinetic parameters of mutants versus native enzyme. The difference was considered statistically significant when p
创建时间:
2015-12-02



