RNA expression at chromosome 8q24 in human colon. Homo sapiens

An inherited variant on chromosome 8q24, rs6983267, is significantly associated with cancer pathogenesis. We present evidence that this region is a transcriptional enhancer, that the risk region physically interacts with the MYC proto-oncogene, and that the alleles of rs6983267 differentially bind transcription factor 7-like 2 (TCF7L2). These data provide strong support for a biological mechanism underlying this non-protein coding risk variant. Overall design: Total RNA and genomic DNA were isolated from colon tissue in 5 individuals. cDNA and DNA were analyzed on separate tracks, with the genomic DNA used to identify sprurious cross-hybridization