Table3_DDX59-AS1 is a prognostic biomarker and correlated with immune infiltrates in OSCC.docx

Background: lncRNAs play a critical role in multiple steps of gene regulation associated with tumor progression. However, the engagement of DDX59-AS1, a lncRNA, remains equivocal, particularly in oral squamous cell carcinoma (OSCC). In this study, the expression of DDX59-AS1 and its association with immune infiltration were investigated, and its prognostic value in OSSC was evaluated.Methods: OSCC patients were collected from The Cancer Genome Atlas (TCGA) database. The expression of DDX59-AS1 in OSCC and healthy tissue was compared using Wilcoxon rank sum test. The relationship between DDX59-AS1 and clinicopathological features was analyzed using Logistic regression. Gene ontology (GO) terminology analysis, gene set enrichment analysis (GSEA), and single sample GSEA (ssGSEA) were utilized to interpret the enrichment pathway and functionality and to quantify the immune cell infiltration of DDX59-AS1. The correlation between survival and DDA59-AS1 was evaluated by Kaplan-Meier analysis and Cox regression. The prognostic impact of DDX59-AS1 was predicted by the nomogram based on Cox multivariate analysis.Results: High expression of DDX59-AS1 was significantly correlated with T stage, clinical stage, race, and age (p < 0.05). Multivariate survival analysis demonstrated that the high expression of DDX59-AS1 was associated with lower overall and specific survival rates. The prognosis prediction was validated by the nomogram and calibration curves. The expression of DDX59-AS1 was negatively correlated with Mast cells, Tfh, T cells, Treg, and B cells, and positively related with the Tgd infiltration level.Conclusion: DDX59-AS1 played a crucial role in the progression and prognosis of OSCC and was potentially a predictive biomarker for OSCC.

背景：长链非编码RNA (lncRNA) 在肿瘤进展过程中与基因调控的多步骤密切相关。然而，DDX59-AS1这一长链非编码RNA的作用尚存在争议，尤其是在口腔鳞状细胞癌 (OSCC) 中。在本研究中，对DDX59-AS1的表达及其与免疫浸润的关系进行了探讨，并评估其在OSCC中的预后价值。方法：从癌症基因组图谱 (TCGA) 数据库中收集OSCC患者样本。利用Wilcoxon秩和检验比较OSCC和健康组织中DDX59-AS1的表达。通过Logistic回归分析DDX59-AS1与临床病理特征之间的关系。采用基因本体 (GO) 术语分析、基因集富集分析 (GSEA) 以及单样本GSEA (ssGSEA) 来解释富集通路和功能，并量化DDX59-AS1的免疫细胞浸润。通过Kaplan-Meier分析和Cox回归评估生存与DDX59-AS1之间的相关性。基于Cox多变量分析的nomogram预测DDX59-AS1的预后影响。结果：DDX59-AS1的高表达与T期、临床分期、种族和年龄显著相关（p < 0.05）。多因素生存分析显示，DDX59-AS1的高表达与较低的总生存率和特异性生存率相关。预后预测通过nomogram和校准曲线得到验证。DDX59-AS1的表达与肥大细胞、Tfh细胞、T细胞、Treg细胞和B细胞呈负相关，与Tgd浸润水平呈正相关。结论：DDX59-AS1在OSCC的进展和预后中起着关键作用，并可能成为OSCC的预测生物标志物。