Transcriptomic profiling of triple-negative breast cancer (TNBC) patient-derived xenografts (PDX) sensitive and resistant to docetaxel.

SurePrint G3 Human Gene Expression Microarray v2 was used to obtain gene expression profiles of two different TNBC PDX models sensitive to docetaxel and in the docetaxel resistant TNBC PDX models derived from both initially sensitive. RNA was isolated from frozen tumors of two different TNBC PDXs models sensitive to docetaxel and from the docetaxel resistant-derived TNBC PDX models. DNAse treated RNA from each sample (n=22) was hybridized to the SurePrint G3 Human Gene Expression Microarray v2 (ID 039494). The Two-Color Microarray-Based Gene Expression Analysis v. 6.5 (Agilent) was used for the labeling.