five

Minimally destructive hDNA extraction method for retrospective genetics of pinned historical Lepidoptera specimens

收藏
DataONE2024-05-22 更新2024-06-08 收录
下载链接:
https://search.dataone.org/view/https://doi.org/10.5061/dryad.pzgmsbcvf
下载链接
链接失效反馈
资源简介:
The millions of specimens stored in entomological collections provide a unique opportunity to study historical insect diversity. Current technologies allow to sequence entire genomes of historical specimens and estimate past genetic diversity of present-day endangered species, advancing our understanding of anthropogenic impact on genetic diversity and enabling the implementation of conservation strategies. A limiting challenge is the extraction of historical DNA (hDNA) of adequate quality for sequencing platforms. We tested four hDNA extraction protocols on five body parts of pinned false heath fritillary butterflies, Melitaea diamina, aiming to minimise specimen damage, preserve their scientific value to the collections, and maximise DNA quality and yield for whole-genome re-sequencing. We developed a very effective approach that successfully recovers hDNA appropriate for short-read sequencing from a single leg of pinned specimens using silica-based DNA extraction columns and an extra..., The draft de novo Melitaea diamina reference genome (butterfly_v1.asm.bp.p_ctg_mtDNA_masked.fa) was based on 6.2 Gb PacBio HiFi reads and assembled and sequenced at the Functional Genomics Center Zurich, FGCZ. The genome was assembled and purged for duplicates with hifiasm (Cheng et al. 2021) and the parameters -l3 -s 0.55. The assembled genome is 805 Mb long and encompasses 3,918 contigs and has a BUSCO (v5.2.2 arthropoda_odb10; (Manni et al. 2021)value of 96.2%. The mtDNA contigs were masked except for one contiguous mtDNA sequence to ensure consistent mapping on mtDNA., , # Data from: Minimally destructive hDNA extraction method for retrospective genetics of pinned historical Lepidoptera specimens [https://doi.org/10.5061/dryad.pzgmsbcvf](https://doi.org/10.5061/dryad.pzgmsbcvf) ***Melitaea diamina*** **draft reference genome assembly** **Martin C. Fischer (ETH Zurich) and Natalia Zajac (Functional Genomic Center Zurich)** The draft *de novo* *Melitaea* *diamina* reference genome (butterfly_v1.asm.bp.p_ctg_mtDNA_masked.fa) was based on 6.2 Gb PacBio HiFi reads and assembled and sequenced at the Functional Genomics Center Zurich, FGCZ. The genome was assembled and purged for duplicates with hifiasm (Cheng* et al.* 2021) and the parameters -l3 -s 0.55. The assembled genome is 805 Mb long and encompasses 3,918 contigs and has a BUSCO (v5.2.2 arthropoda_odb10; (Manni* et al.* 2021)value of 96.2%. The mtDNA contigs were masked except for one contiguous mtDNA sequence to ensure consistent mapping on mtDNA. | **Summery statistics of butterfly\_v1.asm.bp.p...
创建时间:
2024-05-23
用户留言
有没有相关的论文或文献参考?
这个数据集是基于什么背景创建的?
数据集的作者是谁?
能帮我联系到这个数据集的作者吗?
这个数据集如何下载?
点击留言
数据主题
具身智能
数据集  4098个
机构  8个
大模型
数据集  439个
机构  10个
无人机
数据集  37个
机构  6个
指令微调
数据集  36个
机构  6个
蛋白质结构
数据集  50个
机构  8个
空间智能
数据集  21个
机构  5个
5,000+
优质数据集
54 个
任务类型
进入经典数据集
热门数据集

中国交通事故深度调查(CIDAS)数据集

交通事故深度调查数据通过采用科学系统方法现场调查中国道路上实际发生交通事故相关的道路环境、道路交通行为、车辆损坏、人员损伤信息,以探究碰撞事故中车损和人伤机理。目前已积累深度调查事故10000余例,单个案例信息包含人、车 、路和环境多维信息组成的3000多个字段。该数据集可作为深入分析中国道路交通事故工况特征,探索事故预防和损伤防护措施的关键数据源,为制定汽车安全法规和标准、完善汽车测评试验规程、

北方大数据交易中心2024-07-05 收录

LIDC-IDRI

LIDC-IDRI 数据集包含来自四位经验丰富的胸部放射科医师的病变注释。 LIDC-IDRI 包含来自 1010 名肺部患者的 1018 份低剂量肺部 CT。

OpenDataLab2024-05-09 收录

YOLO-dataset

该数据集用于训练YOLO模型,包括分类、检测和姿态识别模型。目前支持v8版本,未来计划支持更多版本。

github2024-10-01 收录

Plant-Diseases

Dataset for Plant Diseases containg variours Plant Disease

kaggle2024-07-13 收录

PDT Dataset

PDT数据集是由山东计算机科学中心(国家超级计算济南中心)和齐鲁工业大学(山东省科学院)联合开发的无人机目标检测数据集,专门用于检测树木病虫害。该数据集包含高分辨率和低分辨率两种版本,共计5775张图像,涵盖了健康和受病虫害影响的松树图像。数据集的创建过程包括实地采集、数据预处理和人工标注,旨在为无人机在农业中的精准喷洒提供高精度的目标检测支持。PDT数据集的应用领域主要集中在农业无人机技术,旨在提高无人机在植物保护中的目标识别精度,解决传统检测模型在实际应用中的不足。

arXiv2024-09-26 收录