Determination of kinact and Ki,app for metabolism-dependent inhibition of CYP3A4 by macrolides.
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Apparent inactivation rates (kobs) were estimated graphically from the slope of the plot for the natural log of enzyme activity remaining after preincubation against preincubation time and were corrected for any loss of activity in the absence of the inhibitor. kinact and Ki,app values were obtained from a double reciprocal plot of kobs against inhibitor concentration. Data represent means ± S.D. of triplicate determinations.
创建时间:
2017-10-25



