Regulatory dynamics determine cell fate following abrupt antibiotic exposure. Schultz et al

Data_S1 is a Matlab file containing the growth curves of six liquid-culture experiments used in Figures 1 and 2, obtained with an automated robotic system at 30ºC. The 'Experiment' field includes a reference to the panels in the main text where the data is used, and the 'Info' field specifies how tetracycline was added to the cultures. The 'Data' field contains optical density (OD) and fluorescence (when applicable) measurements for each strain used in the experiment, together with vectors specifying the gradients of IPTG and tetracycline used (when applicable), the time of the measurements, indication of each type of measurement performed and times of IPTG induction (in Fig. 1F). A detailed description of the methods and strains used is given in the STAR Methods section. Data_S2 contains the image files obtained with the microfluidic device, where cells carrying the native tet resistance mechanism were exposed to a step increase of 70μg/ml tetracycline at time zero. Images were taken every 10 minutes at 10 different positions in 3 different fluorescence channels. CFP was expressed constitutively and used for image segmentation. GFP was expressed from the same promoter as TetR and mCherry was expressed from the same promoter as TetA. The file name indicates the time, position and fluorescence channel. A detailed description of the experiment and the strain used is given in the STAR Methods section.Data_S1 是一个 MATLAB 文件，包含了用于图 1 和图 2 的 6 项液体培养实验的生长曲线，这些数据由自动化机器人系统在 30℃ 下采集得到。其中"Experiment"字段标注了该数据在正文中对应使用的面板编号，"Info"字段说明了四环素向培养液中的添加方式。"Data"字段包含了实验中所用各菌株的光密度(OD)与荧光（若适用）测量值，同时附带了指定 IPTG 与四环素梯度的向量（若适用）、测量时间、所执行的各类测量类型说明，以及 IPTG 诱导的时间点（对应图 1F）。相关实验方法与所用菌株的详细说明见于 STAR Methods 章节。 Data_S2 包含了微流控装置获取的图像文件，该实验中携带天然四环素抗性机制的细胞在时间零点被施加 70μg/ml 四环素的阶跃式浓度提升。图像每 10 分钟采集一次，覆盖 3 个不同荧光通道的 10 个不同位置。青色荧光蛋白(CFP)为组成型表达，用于图像分割；绿色荧光蛋白(GFP)的表达与 TetR 共享同一启动子，mCherry 荧光蛋白的表达则与 TetA 共享同一启动子。文件名标注了采集时间、成像位置与所用荧光通道。相关实验与所用菌株的详细说明见于 STAR Methods 章节。