RT-qPCR data from "The multifaceted routes of host-pathogen interaction mediated by Staphylococcus aureus extracellular vesicles"

All RT-qPCR data were generated from cultured MG-63 cells supplemented, or not, with with 80 µM dynasore, one hour prior to exposure to PBS (control for EV treatment), 0.2% dimethyl sulfoxide (DMSO), 20 µg of EVs in PBS, or 20 µg of EV in PBS with 0.2% DMSO (final concentration) (control for EV treatment in the presence of dynasore). RT-qPCR was conducted using three biological replicates, and three independent technical replicates (n = 9) for each cDNA sample. The amount of mRNA was normalized by using the geometrical mean value of three reference genes, GAPDH, PGK1, and PPIA. Gene expression values were calculated using the 2-ΔΔCT method relative to control conditions (PBS for EV treatment and EVs in PBS-DMSO for EV treatment in the presence of dynasore). Dataset includes raw and normalized RT-qPCR data.