Single-Cell RNA-Seq Analysis Reveals Macrophage Involved in Pathogenesis of Human sporadic type A aortic dissection

Macrophages play an important role in the progression of sporadic acute type A aortic dissection (ATAAD). The aim of this study was to characterize the cellular heterogeneity of macrophages in AD tissues by scRNA-seq. Ascending aortic wall tissue from 6 ATAAD patients and 3 heart transplant donors were assessed by scRNA-seq, then analyzed and validated by various bioinformatics algo-rithms and histopathology experiments. The results revealed the proportion of macrophages in AD tissues (24.51%) was significantly higher than that in normal tissues (13.69%). Among the 6 macro-phage subclusters, pro-inflammatory macrophages accounted for 14.96% in the AD group, and 0.18% in the normal group. Chemokine and inflammation related genes (CCL2, CCL20, S100A8, and S100A9) were expressed more intensively in macrophages in AD tissue than that in the normal tissues. Ad-ditionally, intercellular communication analysis and transcription factor analysis indicated activation of inflammation and degradation of the extracellular matrix in AD tissues. Finally, immunohisto-chemistry, immunofluorescence and western blot experiments confirmed the overexpression of macrophage marker genes (CD68 and CD163) and matrix metalloproteinases (MMP9 and MMP2) in AD tissues. Collectively, our study provides a preliminary evaluation of the role of macrophages in AD and potentially aid in the development of therapeutic options in the future. We performed scRNA sequencing to understand macrophage function in in Pathogenesis of Human sporadic type A aortic dissection. We analyzed and validated by various bioinformatics algorithms and histopathology experiments.

搜集汇总

背景与挑战

背景概述

该数据集通过单细胞RNA测序分析人类散发性A型主动脉夹层组织中的巨噬细胞异质性，发现AD组织中巨噬细胞比例显著升高，促炎性巨噬细胞亚群在AD组中占14.96%，而正常组仅0.18%，且趋化因子和炎症相关基因表达增强。研究揭示了巨噬细胞通过炎症和细胞外基质降解参与主动脉夹层发病机制，为潜在治疗策略提供依据。