DataSheet_9_Integrative analysis of transcriptome, proteome, and ubiquitome changes during rose petal abscission.xlsx

Plant organ abscission is regulated by multiple physiological and biochemical processes. However, the transcriptional, translational, and post-translational modifications occurring during organ abscission have not been systematically investigated. In this study, we report transcriptome, proteome, and ubiquitome data for the abscission zone (AZ) of rose petals collected during petal shedding. We quantified 40,506 genes, 6,595 proteins, and 2,720 ubiquitinated proteins in rose petal AZ. Our results showed that during petal abscission, 1,496 genes were upregulated and 2,199 were downregulated; 271 proteins were upregulated and 444 were downregulated; and 139 ubiquitination sites in 100 proteins were upregulated and 55 ubiquitination sites in 48 proteins were downregulated. Extracellular levels of cell component proteins were significantly increased, while levels within protoplasts were significantly decreased. During petal abscission, transcript levels of genes involved in defense response, transport, and metabolism changed significantly. Levels of proteins involved in the starch and sucrose metabolism and phenylpropanoid biosynthesis pathways were significantly altered at both the transcript and protein levels. The transcriptional and translational upregulation of peroxidase (POD), in the phenylpropanoid biosynthesis, pathway may be associated with deposition of lignin, which forms a protective layer during petal abscission. Overall, our data provide a comprehensive assessment of the translational and post-translational changes that occur during rose petal abscission.

植物器官脱落受多种生理和生化过程调控。然而，在器官脱落过程中发生的转录、翻译以及翻译后修饰尚未得到系统性的研究。在本研究中，我们报告了在花瓣脱落期间收集的玫瑰花瓣脱落区（AZ）的转录组、蛋白质组和泛素化组数据。我们量化了玫瑰花瓣AZ中的40,506个基因、6,595个蛋白质和2,720个泛素化蛋白质。我们的结果显示，在花瓣脱落过程中，1,496个基因上调表达，2,199个基因下调表达；271个蛋白质上调表达，444个蛋白质下调表达；在100个蛋白质中的139个泛素化位点上调表达，而在48个蛋白质中的55个泛素化位点下调表达。细胞外细胞组分蛋白质水平显著增加，而原生质体内的水平则显著降低。在花瓣脱落过程中，参与防御反应、运输和代谢的基因的转录水平发生了显著变化。参与淀粉和蔗糖代谢以及苯丙烷类化合物生物合成途径的蛋白质水平在转录和蛋白质水平上均发生了显著改变。在苯丙烷类化合物生物合成途径中，过氧化物酶（POD）的转录和翻译上调可能与木质素的沉积有关，木质素在花瓣脱落过程中形成保护层。总体而言，我们的数据对玫瑰花瓣脱落过程中的翻译和翻译后变化进行了全面评估。