Spatial and quantitative datasets of the pancreatic β-cell mass distribution in lean and obese mice

A detailed understanding of pancreatic β-cell mass distribution is a key element to fully appreciate the pathophysiology of models of diabetes and metabolic stress. Commonly, such assessments have been performed by stereological approaches that rely on the extrapolation of two-dimensional data and provide very limited topological information. We present ex vivo optical tomographic data sets of the full β-cell mass distribution in cohorts of obese ob/ob mice and their lean controls, together with information about individual islet β-cell volumes, their three-dimensional coordinates and shape throughout the volume of the pancreas between 4 and 52 weeks of age. These data sets offer the currently most comprehensive public record of the β-cell mass distribution in the mouse. As such, they may serve as a quantitative and topological reference for the planning of a variety of in vivo or ex vivo experiments including computational modelling and statistical analyses. By shedding light on intra-...对胰腺β细胞总量（β-cell mass）分布的详尽解析，是全面理解糖尿病模型与代谢应激病理生理学的核心要素。常规此类评估多采用依赖二维数据外推的体视学方法，这类方法所能提供的拓扑学信息极为有限。本研究公开了肥胖型ob/ob小鼠及其瘦型对照组队列中完整胰腺β细胞总量分布的离体光学断层扫描数据集，同时包含4至52周龄期间小鼠全胰腺体积内单个胰岛β细胞的体积、三维坐标与形态学信息。该数据集为当前小鼠胰腺β细胞总量分布领域最全面的公开数据集。因此，本数据集可作为包括计算建模与统计分析在内的各类体内、体外实验规划的定量与拓扑学参考依据。通过阐明胰腺内...