BRB-seq analysis of PFOS disruption of key developmental pathways during hiPSC-derived cardiomyocyte differentiation

Exposure to per- and polyfluoroalkyl substances (PFASs) such as perfluorooctanesulfonic acid (PFOS) has been associated with congenital heart disease (CHD) and decreased birth weight. PFAS exposure can disrupt signaling pathways relevant for cardiac development in stem cell derived cardiomyocyte assays, including PFOS-induced disruption to in vitro cardiac differentiation into contracting spheroids of cardiomyocytes; the PluriBeat assay. Notably, cell line origin can affect how the assay respond to PFAS exposure. Herein, we examine the effect of PFOS on cardiomyocyte differentiation by transcriptomics profiling of two different human induced pluripotent stem cell (hiPSC) lines, to see if they exhibit a common pattern of disruption. Two stages of differentiation, the cardiac progenitor stage (early) and the cardiomyocyte stage (late), were investigated. Medium from hiPSC-derived cardiomyocyte at day 3 (early stage) and day 7 (late stage) in the human cell line BIONi010-C or IMR90-1 was mixed with either DMSO (vehicle) 6.25 µM, 12.5 µM or 25µM of PFOS. >>>Submitter states: For patient privacy concerns we can't make the raw data fully public in GEO<<<