Transcriptomic Profiling of Myeloid Cells in Acetaminophen-Induced Acute Liver Failure

In the recent study, we found that mesenchymal stem cell (MSC)-derived extracellular vesicles expressing the SIRPα protein (SIRP-EVs) were significantly distributed within myeloid cells (CD11b+ cells) and kupffer cells (CD11b+/F4/80+ cells) in acetaminophen (APAP)-induced mouse acute liver failure (ALF) model. Furthermore, SIRP-EVs enhanced the phagocytic activity of macrophages by blocking CD47 on necroptotic hepatocytes and promoted liver regeneration. Therefore, we investigated the therapeutic effects of SIRP-EVs on CD11b+ cells in APAP-induced ALF conditions. CD11b+ cells in the liver, including resident and recruited CD11b+ cells, were harvested and analyzed through bulk RNA sequencing. APAP was administered intraperitoneally to induce ALF, and EVs were administered intravenously 8 hours after APAP induction. 48 hours after APAP induction, livers were harvested and dissociated into single cells. CD11b+ cells were then immediately isolated from liver single-cell suspensions and analyzed via bulk RNA-sequenceing.