RNA-seq Analysis of Prmt1f/f and Lrat-Cre; Prmt1f/f Mouse Fibrotic Liver Transcriptomes

Purpose: To identify the alterations of genes transcriptional profile of livers obtained from Prmt1f/f mice and Lrat-Cre; Prmt1f/f mice treated with thioacetamide (TAA). Methods: Lrat-Cre mice were crossed with and Prmt1f/f mice to generate Lrat-Cre; Prmt1f/f mice. Prmt1f/f mice and Lrat-Cre; Prmt1f/f mice were treated with TAA (400mg/L, drinking water for 16 weeks) to induce liver fibrosis. Liver mRNA profiles of 10-week-old Prmt1f/f and Lrat-Cre; Prmt1f/f mice treated with TAA were generated by deep sequencing, using Illumina Novaseq 6000. We applied DESeq2 algorithm to filter the differentially expressed genes, after the significant analysis and FDR analysis under the following criteria: i) |log2FC| > 1 ; ii) Pvalue < 0.05. RT–PCR validation was performed using SYBR Green PCR Kit. Results: We identified 16916 transcripts in the livers of Prmt1f/f and Lrat-Cre; Prmt1f/f mice. 726 transcripts showed differential expression between Prmt1f/f and Lrat-Cre; Prmt1f/f livers, with a |log2FC| > 1 and P value < 0.05. Altered expression of 4 genes was confirmed by qRT–PCR. Conclusion: Our study represents the first detailed analysis of liver transcriptomes of Lrat-Cre; Prmt1f/f mice during liver fibrogenesis. RNA-seq based transcriptome analysis would provide us better understanding of complex biologic functions of Prmt1 in the hepatic stellate cell during the pricess of hepatic fibrogenesis in mice. Examine the liver mRNA profiles of 10-week old Lrat-Cre; Prmt1f/f mice and Prmt1f/f mice treated with TAA by RNA-seq technolodgy