NextClone and CloneDetective: An Integrated Nextflow Pipeline and R Package for Clonal Barcode Extraction and Quantification

BAM file (chunk 26-50 of 50) for the scRNAseq data required to replicate the analyses presented at: https://phipsonlab.github.io/NextClone-analysis/. Chunk 1-25 can be downloaded from https://zenodo.org/records/10129134. The original BAM file was too big to fit in an entry. Thus it was split into 50 using picard: SplitSamByNumberOfReads -I possorted_genome_bam.bam -O split_sam/ -N_FILES 50 --CREATE_MD5_FILE Before re-running all the analyses in https://phipsonlab.github.io/NextClone-analysis/, make sure you merge all 50 chunks first using picard (change xxx to point to the directory storing all the 50 chunks you have downloaded): outdir="xxx" args="" # Loop through each BAM file for file in ${outdir}/*.bam; do args+="-I $file " done # Do the actual merging MergeSamFiles $args -O $outdir/merged_v2/merged_bam_v2.bam --USE_THREADING --CREATE_MD5_FILE Picard can be downloaded from: https://github.com/broadinstitute/picard