Analysis of differentiatng monocytes transcriptome changes in response to type I IFNs

To analyze the transcriptome changes of differentiating monocytes in response to type I IFNs, RNA seq was performed. The MACS-purified mouse BM monocytes were cultured in the presence or absence of I IFNs for 12 h or 24h under the condition of M-CSF, samples were harvested for total RNAs and subjected to RNA sequencing analyses. Overall design: MACS-purified monocytes from mouse bone marrow were treated with 20 ng/mL M-CSF, together with or without 100U mouse IFNß for 12h, or 24h. each sample had three replicates. Total RNA was harvested at the indicated time, and then the RNA samples were subjected to RNA sequencing analyses.