Supporting data for "Exploring the Cellular and Molecular Basis of Murine Cardiac Development through Spatiotemporal Transcriptome Sequencing"

Spatial transcriptomics is a powerful tool that combines molecular data with spatial information, enabling a deeper understanding of tissue morphology and cellular interactions. In this study, we employed state-of-the-art spatial transcriptome sequencing technology to investigate the development of the mouse heart and establish a comprehensive spatiotemporal cell atlas of early murine cardiac development. Through the analysis of this atlas, we elucidated the spatial organization of cardiac cellular lineages and their interactions during development. Notably, we observed dynamic changes in gene expression within fibroblasts and cardiomyocytes. Furthermore, we identified critical genes including <i>Igf2</i>, <i>H19</i>, <i>Tcap</i>, as well as transcription factors of <i>Tcf12</i> and <i>Plagl1</i>, that may be associated with the loss of myocardial regeneration ability during early heart development. Moreover, we successfully identified marker genes, such as <i>Adamts8</i> and <i>Bmp10</i>, that can distinguish between the left and right atria. Our study provides novel insights into murine cardiac development and offers a valuable resource for future investigations in the field of heart research.