RNA-seq data of esophageal cancer cell Kyse510 after inhibition or silencing of LSD1 and G9a

The goals of this study are to compare transcriptome profiling (mRNA level) and signaling pathways between the control and ablating LSD1, the control and ablating G9a, the control and co-ablating LSD1 and G9a. We found pathways in apoptosis, endoplasmic reticulum stress and unfolded protein response were obviously changed in these groups, which are important for esophageal cancer therapy. Overall design: For inhibition of LSD1 and G9a, Kyse510 esophageal cancer cells were treated with vehicle, 7uM SP2509, 7uM UNC0642 and the combination for 2 days, then whole RNA was extracted and RNA-seq analysis was performed. For silencing of LSD1/G9a, Kyse510 esophageal cancer cells were transfected with sh-LSD1 or sh-G9a and screened 14 days for stable clones through puromycin. Then doxycycline was used to silence LSD1 and G9a for 2 days, then whole RNA was extracted and RNA-seq analysis was performed.