Summary of RNase and nucleic acid binding activity of purified proteins.

aProtein amounts determined by comparative densitometric analysis of Coomassie stained bands in SDS-PAGE gel to a known protein gradient. Nucleic acid amounts determined by spectrophotometric analysis.b“−” denotes little or no activity up to 0.1 µg protein; “+” denotes clearly visible RNA degradation at 0.1 µg protein; “+ +” denotes the most RNA degradation of all proteins.c“none” denotes that with the highest protein amount tested (in brackets), none of the DNA was visibly shifted; “Partial” denotes either the DNA band was visibly more diffuse as compared to the control lanes, or that a distinct band was seen in reactions with protein migrating slower than the unbound DNA; “Complete” denotes that in reactions with the specified amount of protein, no band was seen migrating at the location of the band in the control reaction.dVolumes listed are those seen in Figure 1 (6 L fly coilin culture was used in Figure 1). For GST, the latter volume was used in Figure S3.