A multiscale approach to decipher molecular mechanisms implicated in the intergenerational effect of ibuprofen on the mosquito Aedes aegypti

A laboratory strain of Ae. aegypti (Liverpool strain) was exposed to ibuprofen (1µg/L)from larvae hatching to imagoes emergence. To understand the mechanistic processes underlying the direct and intergenerational impacts of ibuprofen, we used RNA-seq based transcriptomic approach. Each condition (exposed or not to ibuprofen) was analyzed in triplicate, each RNA sample being extracted from 2-4 days old non-blood fed adult females (exposed or not) and from their progeny (L1 larvae exposed or not) . RNA-seq libraries were sequenced on an illumina GAIIx sequencer as 150 bp single reads.