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HDY-12_ORO379_nSSR_data

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DataONE2014-02-07 更新2024-06-27 收录
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资源简介:
Genetic data and spatial coordinates from nine field populations of Ulmus minor Mill. located in Majorca and Minorca Islands (Balearics), Baetic mountains in SE Iberia and Catalonia in NE Iberia. DNA extracted from fresh tissue was amplified by nine microsatellite loci, six of them specifically developed for Ulmus minor (Ulmi1-11, Ulmi1-21, Ulmi1-98, Ulmi1-165, Ulmi2-16 and Ulmi2-20 (Collada et al., 2004)) and three developed for U. laevis (Ulm2, Ulm3 and Ulm8 (Whiteley et al 2003)). A Li-Cor 4300 sequencer (Li-Cor Biosciences, Lincoln, USA) was used to obtain banding profiles. Allele sizes given in base pairs were determined using the SequamarkTM molecular size standard (Invitrogen, Carlsbad, USA) and the software Gene ImagIR 3.56 (Scanalytics).
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2014-02-07
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