Cohesin protein Smc3 influences kinocilial structure and function

Cohesinopathies and ciliopathies are congenital disorders affecting overlapping body systems. The extent to which these syndromes may be linked remains largely untested. Recently, reduced expression of a cohesin core subunit, Smc3, was found to result in abnormal otolith development in zebrafish embryos. This finding suggests that Smc3 may contribute to kinociliary development and function, which would represent a novel role for Smc3. Using hair cells found in neuromasts of the posterior lateral line, we found that Smc3 knockdown resulted in reduced kinociliary length. To address the role of Smc3 in kinocilial function, we monitored neomycin resistance of neuromasts (associated with several cilial gene mutants) and FM1-43X uptake in hair cells (associated with mechanotransduction). We found that Smc3 knockdown indeed led to neomycin resistance of the posterior lateral line neuromasts, suggesting impaired kinocilium function. However, neuromast hair cells did not have defects in FM1-43X ..., , # Data from: Cohesin protein Smc3 influences kinocilial structure and function

Dataset DOI: [10.5061/dryad.5dv41nsk3](https://doi.org/10.5061/dryad.5dv41nsk3)

## Description of the data and file structure

This dataset includes image files captured and quantitative analysis performed to discern the influence of cohesin protein Smc3 on kinociliary structure and/or development in zebrafish larvae. Datasets are organized in separate .zip files for separate experimental efforts corresponding to each figure in the manuscript. Each .zip file contains additional README files with details to provide necessary information.

### Files and variables

#### File: Figure_1_Data.zip

**Description:** This .zip file contains .tiff files of each imaged and evaluated neuromast for kinociliary length analysis. Folder names indicate the injection type. The dataset includes .tiff files of observed neuromasts used for kinociliary length analysis. Each file name corresponds directly to the identifiers used ...,