Preprocessed dataset of "Spatial single-cell multi-omics reveals transcription-translation signatures in mouse brain aging"

Gene expression is regulated at both transcriptional and translational levels, yet current spatial omics technologies typically measure one modality at a time, limiting our ability to deconvolve their respective regulatory contributions at the single-cell level. Here, we introduce qRIBOmap, a new spatial multi-omics platform that enables co-profiling of transcriptome and translatome in the same cells with subcellular resolution. By combining competitive probe hybridization with selective recognition of fully assembled 80S ribosomes, qRIBOmap enables specific detection of translating mRNAs and direct quantification of translation efficiency in intact tissue. Applying qRIBOmap to brain tissue from young and aged mice, we co-profiled 2,043 genes and uncovered widespread cell type–specific remodeling of translational regulation during aging, including altered subcellular organization of translation in neurons and glia. These results reveal new molecular signatures in brain aging and establish qRIBOmap as a powerful tool for spatially resolved interrogation of gene regulation.