Transcriptional responses in a mouse model of silicone wire embolization induced acute retinal artery Ischemia and Reperfusion

Acute retinal ischemia and ischemia-reperfusion injury are the primary causes of retinal neural cell death and vision loss in retinal artery occlusion (RAO). The absence of an accurate mouse model for simulating the retinal ischemic process has hindered progress in developing neuroprotective agents for RAO. We developed a unilateral pterygopalatine ophthalmic artery occlusion (UPOAO) mouse model using silicone wire embolization combined with carotid artery ligation. The survival of retinal ganglion cells and visual function were evaluated to determine the duration of ischemia. Immunofluorescence staining, optical coherence tomography, and haematoxylin and eosin staining were utilized to assess changes in major neural cell classes and retinal structure degeneration at two reperfusion durations. Transcriptomics was employed to investigate alterations in the pathological process of UPOAO following ischemia and reperfusion, highlighting transcriptomic differences between UPOAO and other ret..., Retinal samples from UPOAO, HIOP, and UCCAO mice were extracted and promptly frozen in liquid nitrogen within 10 minutes of cervical dislocation. Total RNA extraction was performed according to the protocol outlined in the TRIzol Reagent manual (Life Technologies, CA, USA). RNA integrity and concentration were assessed using an Agilent 2,100 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA, USA). The resulting RNA samples were then pooled and subjected to sequencing on the Illumina HiSeq3000 platform in a 150-bp paired-end read format. The raw RNA-sequencing (RNA-seq) reads were preprocessed and quantified using the featureCounts function in the SubReads package version 1.5.3, with default parameters.
RNA-seq analysis was conducted on samples from 5 mice in the non-perfusion group, 4 mice in the 3-days perfusion group, and 5 mice in the 7-days perfusion group of the UPOAO model. Additionally, retinas from both the 7-days HIOP experimental eyes and bilateral UCCAO eyes were colle..., , # Transcriptional Responses in a Mouse Model of Silicone Wire Embolization Induced Acute Retinal Artery Ischemia and Reperfusion

[https://doi.org/10.5061/dryad.s1rn8pkhb](https://doi.org/10.5061/dryad.s1rn8pkhb)

## Description of the data and file structure

### Files and variables

#### File: Representation\_matrix\_data.rar

**Description:** 

1. Filename: HIOP_7d      Description: Transcriptome data at 7 days in mice with high intraocular pressure model.     HIOP_1 - HIOP_5: Mice with high intraocular pressure model.  Sham_1 - Sham_5: Sham operation group mice.
2. Filename: UCCAO_7d    Description: Transcriptome data at 7 days in mice with unilateral common carotid artery occlusion model.      UCCAO_1-UCCAO_5: Mice with unilateral common carotid artery occlusion model.  Sham_1 - Sham_5: Sham operation group mice.
3. Filename: UPOAO    Description: Unilateral pterygopalatine ophthalmic artery occlusion model transcriptome data taken immediately after modeling.   UPOAO1-UPOAO_5: Mice...