Serotypes and genotypic characteristics of EHEC strains isolated from patients with HUS in the Czech Republic, 1998–2012.

aThe genes encode the following proteins: fliC, flagellar subunit of H antigen; stx, Shiga toxin; cdt-V, cytolethal distending toxin V; EHEC-hlyA, EHEC hemolysin; α-hlyA,α hemolysin; espP, serine protease EspP; eae, intimin; efa1, EHEC factor for adherence; lpfAO26, major subunit of long polar fimbriae of EHEC O26; lpfA O157/OI-141 and lpfAO157/OI-154, major subunit of long polar fimbriae of EHEC O157 encoded on O island OI 154 and OI 141, respectively; iha, iron-regulated gene A homologue adhesin; terE, marker for tellurite resistence-encoding cluster; ureD, marker for ure cluster encoding urease production; irp2 and fyuA, markers for the high pathogenicity island (HPI) encoding iron uptake system.bSerotypes were determined using conventional and molecular serotyping; the fliC genes indicated were present in both motile and non-motile strains of each respective serotype; NSF, non-sorbitol-fermenting; SF, sorbitol-fermenting.c−, the gene was absent; +, the gene was present (if the gene was not present in all strains of the respective serotype, the numbers of positive strains are indicated in parenthesis).done strain lost stx gene before subtyping.eespP subtypes are indicated in parentheses.feae subtypes are indicated in parentheses.ga truncated efa1 gene [34] was present in EHEC O157:H7 and one O145:H28 isolate; complete efa1 was present in all other strains.