Hedgehog-regulated genes in the chick coelomic epithelium. Hedgehog-regulated genes in the chick coelomic epithelium
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In the experimental group, the coelomic cavity of HHStage 12-13 chick embryos was electroporated with a plasmid expressing PatchedDLoop2 (a dominant negative hedgehog pathway inhibitor) and GFP. In the control group, a plasmid expressing GFP alone was electroporated. After 14 hours, embryos were dissociated into single cells, FACS sorted, and processed for RNAseq. Overall design: 5 experimental and 4 controls were analyzed. These were all separate biological replicates.
创建时间：
2020-03-25
