Day-night rhythm in murine retinal pigment epithelium. Day-night rhythm in murine retinal pigment epithelium

Murine RPE transcriptomes were generated for 6 time points during a 24 hour period. Our data demonstrates that genes involved in ATP generation have a high expression at night whereas genes involved in β-oxidation and cholesterol synthesis show a high expression in the morning. Additionally, genes were identified in the glycerophospholipid metabolism pathway, suggesting the generation of glycerophospholipids at night. Finally, genes involved in intracellular trafficking with different expression patterns during the day-night cycle were identified and we identified genes involved in actin cytoskeleton building, remodelling and crosslinking with a high expression in the morning. Overall design: Murine RPE transcriptomes were generated for 6 time points during a 24 hour period (ZT0, 2, 4, 9, 14, 19). In total 30 samples were sequenced because each time point was repeated 5 times (n=5).