Analysis of gene expression changes upon ZBTB18 full length (FL), ZBTB18 full length-mutant (FL-mut), ZBTB18 short form Nte (SF-Nte) and ZBTB18 short form Nte (SF-mut) in SNB19 glioblastoma cell line

ZBTB18 is a transcriptional repressor and tumor suppressor in glioblastoma (GBM). Specifically, it acts as a repressor of mesenchymal genes. ZBTB18 interacts with C-terminal binding proteins 1 and 2 through a VLDL motif. In GBM, ZBTB18 is cleaved the the calpain 2 intracellular proteases which generates truncated Nte and Cte short forms. Here we have investigated gene expression changes which occur upon the expression of ZBTB18 full lenght or ZBTB18 short form Nte (SF-Nte), as well as upon expression of the mutated counterparts which no longer interacts with CTBP1/2. Overall design: mRNA profiles of SNB19 cells transduced with the following lentiviral vectors: pCHMWS (empty vector, EV), pCHMWS-ZBTB18 FL, pCHMWS-ZBTB18 FL-mut, pCHMWS-ZBTB18 SF-Nte, pCHMWS-ZBTB18 SF-mut.