Identification of exosomal miRNAs specifically derived from intrafollicular cells involved in polycystic ovary syndrome. Identification of exosomal miRNAs specifically derived from intrafollicular cells involved in polycystic ovary syndrome

Exosomes have recently been shown to play a key role in cell-to-cell communication through delivery of various functional content, including microRNAs (miRNAs). We investigated the potential roles of exosomal miRNA derived intrafollicular cells in polycystic ovary syndrome (PCOS). Using microarray profiling, a total of 492 miRNAs and 220 miRNAs were found in follicular fluid-derived exosomes and serum-derived exosomes, respectively, in PCOS and non-PCOS females. By excluding miRNAs existing in serum-derived exosomes, we found 179 miRNAs which were specifically expressed in follicular fluid-derived exosomes both in PCOS and non-PCOS females. Using microarray profiling, a total of 492 miRNAs and 220 miRNAs were found in follicular fluid-derived exosomes and serum-derived exosomes, respectively, in PCOS and non-PCOS females. By excluding miRNAs existing in serum-derived exosomes, we found 179 miRNAs which were specifically expressed in follicular fluid-derived exosomes both in PCOS and non-PCOS females. Overall design: Exosomes were isolated from pooled follicular fluid of PCOS and non-PCOS females, respectively. Serum-derived exosomes were isolated from pooled serum of PCOS and non-PCOS females, respectively. miRNA profiles were generated using microarray. Comparison of miRNAs expression in fluid-derived exosomes and serum-derived exosomes were preformed to identify the exosomal miRNAs specifically derived from intrafollicular cells