NanoSINC-seq. Homo sapiens

Transcripts are synthesized in the nucleus and then exported to the cytoplasm, where translation occurs. Alternative isoform usage in the cytoplasm is essential to diversify the protein molecules even in isogenic populations of cells. To obtain a comprehensive understanding of isoform localization and the usage, we here leveraged the physical fractionation of cytoplasmic RNAs versus nucleus via a microfluidic approach coupling with Oxford Nanopore Technologies (ONT) long-read sequencing (NanoSINC-seq). Leveraging the NanoSINC-seq approach, we investigated the difference in transcript usage between nucleus and cytoplasm in leukemic cells (K562).