A vaccine-induced public antibody protects against SARS-CoV-2 and emerging variants

These are the processed BCR repertoire bulk sequencing data described in Schmitz, Turner & Liu et al., Immunity, 2021. The raw sequence data are available on SRA under BioProjects PRJNA731610 and PRJNA741267. 

Summary: Bulk-sorted total plasmablasts and IgDlo enriched B cells from PBMCs and germinal centre B cells from lymph nodes from various timepoints after primary immunization from 22 BNT162b2 vaccinees who had no prior history of infection with SARS-CoV-2. 

Metadata file: WU368_schmitz_et_al_immunity_2021_meta.tsv

Abbreviations:

LN = lymph node

PB = plasmablast

GC = germinal center

mAb = monoclonal antibody

BCR data file: WU368_schmitz_et_al_immunity_2021_bcr.tsv.gz

In addition to the processed bulk sequences, also included are the heavy chains of 37 mAbs (including 2C08) first reported in Turner & O'Halloran et al., Nature, 2021 that had been validated to be spike-binding. The mAbs are annotated as "mab" in the "seq_type" column.

Sequence data column description

The columns largely follow the AIRR-C Rearrangement format. The main deviation is that CDR3s are used, as opposed to IMGT-defined "junctions". Non-standard columns are noted below.

v_call_genotyped: V gene annotation reassigned after individualized genotyping by TIgGER

isotype: IGH[ADEGM]

cdr3: CDR3 nucleotide sequence

cdr3_length: CDR3 nucleotide sequence length

cdr3_aa: CDR3 amino acid sequence

donor: vaccinee ID

sample: sample ID (arbitrary)

timepoint: time point at which sample was collected

tissue: tissue from which sample was collected

sorting: FACS sorting

seq_type: sequence type (mAb or bulk)