Microarray Expression Profiling of Punicalagin, Myricetin and Quercetin Treated Leukemia Cell Line (THP-1)

THP-1 monocyte were differentiated to macrophage using PMA for 24 hrs, then (THP-1) macrophage were treated with DMSO, Punicalagin, Myricetin or Quercetin for 24 hrs. Transcriptional expression profiling was performed with Affymetrix GeneChips. Target preparation, hybridization, washing and staining, and probe array scanning were done to process array. Three different passages of THP-1 monocyte were used 6, 7, and 8. THP-1 moncyte were differentiated to macrophage using PMA for 24 hrs. THP-1 macrophage were treated with DMSO, Punicalagin, Myricetin or Quercetin for 24 hrs.