Single cell transcriptomic profiling of large intestinal enteroendocrine cells in mice

We report the different enteroendocrine populations in the large intestine in mice based on their transcriptomics profile. Hormone, receptor and transcription factors are analysed for differential expression between the different cell clusters to give a comprehensive view of the different characterisitcs of the distal entereondocrine cells Large intestine epithelium from a Neurod1-crexEYFP mouse was digested into single cells and sorted based on EYFP fluorescence to collect only the enteroendocrine cells. 3500 cells were collected and their transcriptome sequenced using the Chromium system (10x Genomics) and a Hiseq4000 sequencer. Analysis of their transcriptome was perfomed using the cellranger pipeline