The different substrates used as carbon source in lipid production media.

Notes:[1]. The corn stover (33A14) used in this study was harvested from the Kramer farm in Wray, Colorado. The milled corn stover powders were treated in the 4-L NREL Steam Gun reactor. The pretreatment conditions are: 160°C, 5 min, 2.0 wt% sulfuric acid. The composition of dilute acid-pretreated corn stover (DAPCS) was: Glucan 60.2%, xylan 4.8%, lignin 24.0%, galactan 0.7%, arabinan 2.1%.[2]. Pre-saccharifications of Avicel or DAPCS were carried out as below: Starting from 2×4.49% (w/v) pretreated corn stover or 2×2.7% (w/v) Avicel by incubation for 3 days with a commercial “cellulase” (GC 220, Genecor/Danisco), loaded at 40 mg protein per g of glucan, at 50°C and pH 4.8 in 50 mM sodium citrate. The pre-saccharification mixture was boiled for 10 min, and then mixed with 2× lipid production (LP) medium to start the culturing.