RNA-seq analysis of HeLa cells overexpressing histone methyltransferase KMT2B

KMT2B,a major H3K4 tri-methyltransferase, contributes to the development of various cancers. However, its role in cervical cancer is unclear. We found that KMT2B is upregulated in cervical cancer tissues, which is significantly associated with poor prognosis.To investigate the underlying mechanism of KMT2B in the progression of cervical cancer, we established stable KMT2B-overexpression HeLa cells.Then we compared the whole genome mRNA expression profile of KMT2B overexpression and control HeLa cells using RNA-seq. Overall design: HeLa cells endogenous overexpressing KMT2B was established by CRISPR/dCas9 synergistic activation mediator (SAM) system. mRNA profiles of the KMT2B-overexpressing Hela cells and control Hela cells were performed by deep sequencing in quadruplicate, using an Illumina, HiseqXTen platform.