Ame-miR-2161 affects the survival and development of honeybee larvae through the juvenile hormone acid methyltransferase gene

MicroRNAs (miRNAs), as endogenous small non-coding RNAs, play a role in regulating the growth and development processes of insects. However, there are still few reports on the coordinated regulation of honeybee development processes by miRNAs and hormones. In this study, the regulatory network of target genes of Apis mellifera miRNA-2161 (ame-miR-2161) was constructed. The association between ame-miR-2161 and the survival and development of workers larvae was analyzed. The results showed that ame-miR-2161 potentially targets 22 mRNAs, focusing on target gene juvenile hormone acid methyltransferase (Jhamt), which is a key rate-limiting enzyme in the final step of the juvenile hormone synthesis pathway. RT-qPCR revealed that the expression of ame-miR-2161 and Jhamt had the same trend during the larval stage. Dual-luciferase assays confirmed the targeting relationship between ame-miR-2161 and Jhamt. Overexpression of ame-miR-2161 increased the mRNA level of Jhamt, which led to significantl..., Experimental Procedures
Honeybee larvae rearing
The A. mellifera worker larvae were reared in the apiary of the bee protection group at the College of Bee Science and Biomedicine, Fujian Agriculture and Forestry University. We selected Apis mellifera ligustica colonies with strong colony vigor and healthy individual bees. The queen was restricted from laying eggs in the empty comb for 6 h and then removed. After the eggs hatched, 2-day-old larvae (determined by size) were transferred onto a 6-well plate using a clean grafting needle and fed with 50 μL of pre-prepared artificial diet (63% royal jelly, 30% purified water, 6% honey, and 1% yeast extract), then reared them under conditions of 35±0.5°C and 90% relative humidity (RH). Uniform-sized 3-day-old larvae were selected and individually transferred to a 48-well plate for cultivation, with the artificial diet replaced every 24 hours.
Sample collection and RNA extraction
The 4- to 6-day-old worker larvae were collected (three larvae as..., # Data from: Ame-miR-2161 affects the survival and development of honeybee larvae through the juvenile hormone acid methyltransferase gene

Dataset DOI: [10.5061/dryad.44j0zpcsc](10.5061/dryad.44j0zpcsc)

## Description of the data and file structure

We have submitted our raw data. We organized the information in the order shown in the figure, including the original gel image (Fig. 1A), the results of gene expression detection, as well as the survey data such as survival rate (%) and body weight (Unit: g).

### Descriptions

Name: Fig.1A Original image mir2161

* The original gel image of ame-miR-2161

Name: Fig.1B all targets and 22 targets

* All the target genes of ame-miR-2161 and the top 22 selected target genes

Name: Fig. 2A The expression level of miR2161

* The expression level of ame-miR-2161

Name: Fig. 2B The expression level of jhamt

* The expression level of juvenile hormone acid methyltransferase(jhamt)

Name: Fig. 2C Jhant-wt and Fig. 2C Jhant-mutant

* The dual lucif...,