Date fruits inhibit hepatocyte apoptosis and modulate the expression of hepatocyte growth factor, cytochrome P450 2E1 and heme oxygenase-1 in carbon tetrachloride-induced liver fibrosis

<i>Context</i>: Date fruits have protective effects against liver fibrosis; however their anti-apoptotic effects have not been investigated. <i>Objective</i>: To investigate the modulating effects of date fruits on pro- and anti-apoptotic markers, cytochrome P450 2E1 (CYP2E1) and hepatocyte growth factor (HGF) in liver fibrosis. <i>Materials and methods</i>: Liver fibrosis was induced by injection of carbon tetrachloride (CCl₄) for eight weeks. Date flesh extract (DFE) and pits extract (DPE) were taken daily concomitant with CCl₄. Hepatocyte apoptosis was determined by measuring the expression of Fas, caspase-3, Bax, Bcl2 and hemeoxygenase-1 (HO-1). Hepatic levels of HGF and CYP2E1 were determined. <i>Results</i>: Treatment with DFE and DPE significantly attenuated the elevated levels of Fas, caspase 3, Bax and CYP2E1 induced by CCl₄. In addition, they alleviated the reduction in Bcl2, HGF and HO-1, the cytoprotective and anti-apoptotic factors in liver. <i>Conclusions</i> DFE and DPE treatment can ameliorate liver fibrosis by inhibiting hepatocyte apoptosis.