Effect of silencing of DEPDC1 on gene expression in MDA-MB 231 TNBC cell line

DEPDC1 supports tumor growth, migration and metastases formation. To investigate what is the gene expression network regulated by DEPDC1, we silenced its expression in MDA-MB-231 Triple Negative Breast Cancer (TNBC) cell line. We then performed gene expression profiling analysis using data obtained from RNA-seq of MDA-MB 231. Overall design: Comparative gene expression profiling analysis of RNA-seq data for MDA-MB 231 cells transfected with Control or DEPDC1 siRNAs, with 3 and 4 replicates respectably.